Rhizobium sophorae is the dominant rhizobial symbiont of Vicia faba L. In North China |
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| Affiliation: | 1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province 450000, PR China;2. Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou 450002, Henan Province, PR China;3. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China;4. LSTM, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France;5. Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand;6. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México, D. F, Mexico;1. National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment of Shandong Agricultural University, Taian 271000, China;2. Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;3. College of Life Science, Yantai University, Yantai 264005, China;4. College of Marine Science, Shandong University at Weihai, Weihai 264209, China;5. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico;1. Laboratory of Biodiversity and Valorization of Arid Areas Bioresources (BVBAA) – Faculty of Sciences of Gabes, Erriadh, Zrig 6072, Tunisia;2. Centro de Biotecnología y Genómica de Plantas (UPM-INIA), ETSI Agronómica, Alimentaria y de Biosistemas, Campus de Montegancedo, Universidad Politécnica de Madrid, Madrid, Spain;1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People’s Republic of China;2. School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, People’s Republic of China;1. Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakech, Morocco;2. Technische Universität Dresden, Institut für Genetik, Helmholtzstr. 10, D-01069 Dresden, Germany;3. Centre Régional de la Recherche Agronomique de Meknès, INRA, Morocco;4. Department of Biology, Faculty of Science and Techniques, BP. 509, 52000 Boutalamine, Errachidia, Morocco |
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| Abstract: | Faba bean (Vicia faba L.) is a major introduced grain-legume crop cultivated in China. In this study, rhizobia that nodulated faba bean grown in soils from three sites in North China (Hebei Province) were isolated and characterized. Firstly, isolates were categorized into genotypes by ribosomal IGS PCR-RFLP analysis, then representatives of the different IGS genotypes were further identified by phylogenetic analyses of 16S rRNA, housekeeping (atpD, recA) and nodulation (nodC) gene sequences. Rhizobial distribution based on the IGS genotype was related to the different soil physicochemical features by redundancy analysis. IGS typing and phylogenetic analyses of 16S rRNA and concatenated housekeeping gene sequences affiliated the 103 rhizobial strains isolated into four Rhizobium species/genospecies. A total of 69 strains of 3 IGS types were assigned to R. sophorae, 20 isolates of 5 IGS types to R. changzhiense and 9 isolates of 3 IGS types to R. indicum. The representative strain of the five remaining isolates (1 IGS type) was clearly separated from all Rhizobium type strains and was most closely related to defined genospecies according to the recently described R. leguminosarum species complex. Rhizobium sophorae strains (67% of total isolates) were common in all sites and shared an identical nodC sequence typical of faba bean symbionts belonging to symbiovar viciae. In this first study of rhizobia nodulating faba bean in Hebei Province, China, R. sophorae was found to be the dominant symbiont in contrast to other countries. |
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| Keywords: | Faba bean Symbiosis Genetic diversity Rhizobia |
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