Role of propylamine transferases in hormone-induced stimulation of polyamine biosynthesis |
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| Authors: | S G Hughes T Bruce K Murray |
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| Affiliation: | Department of Biochemistry, University of Helsinki, SF-00170 Helsinki 17, Finland |
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| Abstract: | An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments. |
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