Lysine-ketoglutarate reductase activity in developing maize endosperm |
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| Authors: | Arruda P Sodek L da Silva W J |
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| Affiliation: | Department of Genetics and Evolution, Instituto de Biologia, Universidade Estadual de Campinas, 13100 Campinas, SP., Brazil. |
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| Abstract: | Lysine-ketoglutarate reductase activity was detected and characterized in the developing endosperm of maize (Zea mays L.). The enzyme showed specificity for its substrates: lysine, α-ketoglutarate, and NADPH. Formation of the reaction product saccharopine was demonstrated. The pH optimum of the enzyme was close to 7, and the Km for lysine and α-ketoglutarate were 5.2 and 1.8 millimolar, respectively. |
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