Biosynthesis and accumulation of a medicinal compound,Picroside-I,in cultures of <Emphasis Type="Italic">Picrorhiza kurroa</Emphasis> Royle ex Benth |
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Authors: | Hemant Sood R S Chauhan |
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Institution: | (1) Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, 173 215, HP, India; |
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Abstract: | Establishment of callus cultures and plant regeneration from different explants coupled with estimation of Picrosides in morphogenetically
different developmental stages showed that Picroside-I accumulates in shoot cultures of Picrorhiza kurroa with no detection of Picroside-II. The Picroside-I content was 1.9, 1.5, and 0.04 mg/g in leaf discs, stem and root segments,
respectively. The Picroside-I content declined to almost non- detectable levels in callus cultures derived from leaf discs,
stem segments with no change in Picroside-I content in root segments or calli derived thereof. The biosynthesis and accumulation
of Picroside-I started in callus cultures differentiating into shoot primordia and reached to the concentrations comparable
to original explants of leaf discs and stem segments in fully developed shoots with contents of 2.0 and 1.5 mg/g, respectively.
The shoots formed from root-derived callus cultures were relatively slow in growth as well as the amount of Picroside-I content
was comparatively low (1.0 mg/g) compared to shoots derived from callus cultures of leaf and stem segments, respectively.
The current study concludes that the biosynthesis and accumulation of Picroside-I is developmentally regulated in different
morphogenetic stages of P. kurroa tissue cultures. |
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