Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis |
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| Authors: | Boris Wilmes Angelika Hartung Michael Lalk Manuel Liebeke Thomas Schweder Peter Neubauer |
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| Affiliation: | 1.Institute of Marine Biotechnology,Greifswald,Germany;2.Pharmaceutical Biotechnology, Institute of Pharmacy,Ernst-Moritz-Arndt-University,Greifswald,Germany;3.Bioprocess Engineering Laboratory,University of Oulu,Oulu,Finland;4.Department of Internal Medicine IV,University of Tübingen,Tübingen,Germany;5.Competence Center - Functional Genomics,Institute of Pharmacy, Ernst-Moritz-Arndt-University,Greifswald,Germany;6.Biomolecular Medicine, Department of Surgery and Cancer,Faculty of Medicine, Imperial College London,London,UK;7.Laboratory of Bioprocess Engineering, Department of Biotechnology,Technical University Berlin,Berlin,Germany |
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| Abstract: | Background Pseudoalteromonas haloplanktis is a cold-adapted γ-proteobacterium isolated from Antarctic sea ice. It is characterized by remarkably high growth rates at low temperatures. P. haloplanktis is one of the model organisms of cold-adapted bacteria and has been suggested as an alternative host for the soluble overproduction of heterologous proteins which tend to form inclusion bodies in established expression hosts. Despite the progress in establishing P. haloplanktis as an alternative expression host the cell densities obtained with this organism, which is unable to use glucose as a carbon source, are still low. Here we present the first fed-batch cultivation strategy for this auspicious alternative expression host. |
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