甘蔗过氧化氢酶基因的电子克隆及生物信息学分析

应用电子克隆技术,获得甘蔗中一个过氧化氢酶基因的cDNA序列全长,命名为S-CAT。该基因全长2160bp,包含一个1479bp的开放阅读框,编码492个氨基酸。通过PSORT工具,预测甘蔗S-CAT蛋白存在于过氧化物酶体中。同源比对分析显示,S-CAT基因编码的氨基酸序列与水稻、玉米、高粱、朝鲜碱茅、葡萄等植物中过氧化氢酶基因所编码的氨基酸序列高度同源,同源性分别为97%,97%,95%,91%和92%。研究结果为该基因的实验克隆奠定基础。

Electronic cloning and characterization of CAT gene from Saccharum officinarum using bioinformatics tools

A catalase gene from Saccharum officinarum,termed as S-CAT,was cloned in silico based on the corresponding EST sequences from sugarcane EST database in NCBI.Several characters of the amino acid encoded by S-CAT gene were analyzed and predicted by bioinformatics tools,including the composition of amino acid sequence,hydrophobicity and hydrophilicity,secondary structure of protein and so on.Bioinformatics analysis also revealed that the full-length of S-CAT gene from S.officinarum was 2 160 bp,containing a complete ORF encoding 492 amino acids.Using PSORT,it was predicted that the S-CAT protein was located in peroxisome and belonged to the CAT-POD type of catalases.Homology comparison and phylogenetic analysis showed that the amino acid encoded by S-CAT gene in sugarcane was highly homologous with those encoded by CAT gene in rice,maize,sorghum,Korean alkaligrass and grape,with homologies of 97 %,97 % 95 %,91 % and 92 %,respectively.The above results will provide the basis for molecular cloning of CAT genes in sugarcane.