| Vitex glabrata R.Br.悬浮培养细胞的生长条件优化及20-羟基蜕皮激素的生产 |
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| 引用本文： | 张玉霞. Vitex glabrata R.Br.悬浮培养细胞的生长条件优化及20-羟基蜕皮激素的生产[J]. 生物工程学报, 2007, 23(6): 1033-1036. DOI: 10.1016/S1872-2075(07)60061-2 |
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| 作者姓名： | 张玉霞 |
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| 作者单位： | Department of Biotechnology Faculty of Technology，Khon Kaen University，Department of Biotechnology，Faculty of Technology，Khon Kaen University，Department of Biotechnology，Faculty of Technology，Khon Kaen University，Khon Kaen 40002 Thailand，Khon Kaen 40002 Thailand，Khon Kaen 40002 Thailand |
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| 摘 要： | 研究了培养基、植物生长调节素以及接种量对Vitex glabrata R.Br.悬浮培养细胞的生长情况以及对20-羟基蜕皮激素形成的作用。当细胞在添加有2.0mg/LBAP(6-苯甲酸嘌呤)和1.0mg/L2,4-D的Gamborg’s B5培养基中培养时细胞生长和20-羟基蜕皮激素的形成达到了最高水平。当接种量为20%PCV(积压细胞体积)时观察到了20-羟基蜕皮激素的最高产量,大约是1.1mg/(L.d)。实验数据也表明当接种量增加到20%PCV时,产量提高了7倍。
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| 关 键 词： | 20-羟基蜕皮激素 悬浮培养 |
| 文章编号： | 1000-3061(2007)06-1033-04 |
| 收稿时间： | 2007-01-05 |
| 修稿时间： | 2007-01-05 |
Optimization of Cell Growth and 20-hydroxyecdysone Production in Cell Suspension Culture of Vitex glabrata R.Br. |
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| Sinlaparaya Duangjai, Duanghaklang Preeyada,Panichajakul Sanha,. Optimization of Cell Growth and 20-hydroxyecdysone Production in Cell Suspension Culture of Vitex glabrata R.Br.[J]. Chinese journal of biotechnology, 2007, 23(6): 1033-1036. DOI: 10.1016/S1872-2075(07)60061-2 |
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| Authors: | Sinlaparaya Duangjai Duanghaklang Preeyada Panichajakul Sanha |
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| Affiliation: | Department of Biotechnology,Faculty of Technology,Khon Kaen University,Khon Kaen 40002 Thailand |
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| Abstract: | The effects of the cultivation media, plant growth regulators and inoculum size on the cell growth and 20-hydroxyecdysone production in suspension cultures of Vitex glabrata R.Br. were investigated. The cell growth and 20-hydroxyecdysone formation reach the highest when cells are cultured in the Gamborg's B5 medium supplemented with 2.0mg/L BAP (6-benzylaminopurine) and 1.0mg/L 2,4-D. The maximum 20-hydroxyecdysone productivity, of about 1.1mg/L/day, was observed in the culture with 20% PCV (packed cell volume) of inoculum size. These data also show that the increment of the inoculum size to 20% PCV could increase the productivity in 7-folds. |
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| Keywords: | Vitex glabrata |
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