A reference map of the membrane proteome of Enterococcus faecalis |
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Authors: | Maddalo Gianluca Chovanec Peter Stenberg-Bruzell Filippa Nielsen Hailyn V Jensen-Seaman Michael I Ilag Leopold L Kline Kimberly A Daley Daniel O |
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Affiliation: | Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden. |
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Abstract: | Enterococcus faecalis is a gram-positive bacterium that is part of the indigenous microbiotica of humans and animals as well as an opportunistic pathogen. In this study, we have fractionated the membrane proteome of E. faecalis and identified many of its constituents by mass spectrometry. We present blue native-/SDS-PAGE reference maps that contain 102 proteins. These proteins are important for cellular homeostasis, virulence, and antibiotic intervention. Intriguingly, many proteins with no known function were also identified, indicating that there are substantial gaps in the knowledge of this organism's biology. On a more limited scale, we also provide insight into the composition of membrane protein complexes. This study is a first step toward elucidating the membrane proteome of E. faecalis, which is critical for a better understanding of how this bacterium interacts with a host and with the extracellular milieu. |
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