Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae |
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Authors: | Xiaolin Liao Dan Wang Xiaomu Yu Weitao Li Lei Cheng Jianwei Wang Jingou Tong |
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Affiliation: | (1) The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, P. R. China;(2) The Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P. R. China |
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Abstract: | Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in a test population with alleles ranging from 2–7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679 to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely related species for the conservation purpose. |
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Keywords: | Gobiocypris rarus Microsatellite Genetic diversity Cross-species amplification Conservation genetics |
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