Purification and structural characterization of glycolipid biosurfactants fromPseudomonas aeruginosa YPJ-80 |
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| Authors: | Oh-Jin Park Young-Eun Lee Joong-Hoon Cho Hyun-Jae Shin Byung-Dae Yoon Ji-Won Yang |
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| Institution: | (1) BioProcess Engineering Research Center, Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 373-1, Kusung-dong, Yusung-gu, 305-701 Taejon, Korea;(2) Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, Yusung, P.O. Box 115, 305-600 Taejon, Korea |
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| Abstract: | Glycolipids produced byPseudomonas aeruginosa YPJ-80 were characterized by chromatographic and spectroscopic techniques as a mixture of two rhamnolipids. For recovery
of glycolipids from the culture broth, various isolation methods including ultrafiltration, adsorption and solvent extraction
were compared. Ultrafiltration showed the best results in terms of glycolipids recovery. Further purification for spectroscopic
analysis was carried out by adsorption chromatography and preparation thin layer chromatography. From the spectroscopic analysis,
such as IR spectroscopy, FAB-MS,1H-NMR and13C-NMR and hydrolysis analysis, the glycolipids were identified as L-α-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate
and 2-O-α-L-rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate. Monorhamnolipid and dirhamnolipid lowered
the surface tension of water to 28.1 mN/m and 29.3 mN/m, respectively. |
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| Keywords: | Pseudomonas glycolipid biosurfactant emulsification |
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