Genome-wide target profiling of piggyBac and Tol2in HEK 293: pros and cons for gene discovery and gene therapy |
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| Authors: | Yaa-Jyuhn J Meir Matthew T Weirauch Herng-Shing Yang Pei-Cheng Chung Robert K Yu Sareina C-Y Wu |
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| Affiliation: | (1) Department of Biomedical Sciences, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan;(2) Banting and Best Department of Medical Research and Donnelly Centre for Cellular and Biomolecular Research University of Toronto, 112 College Street, Toronto, Ontario, M5 S 3E1, Canada;(3) YongKang Veterans Hospital, 427 Fuxing Road, Yongkang City, Tainan, 71051, Taiwan;(4) Molecular Medicine Research Center, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan 333, Taiwan;(5) Institute of Molecular Medicine and Genetics and Institute of Neuroscience, Georgia Health Sciences University, 1120 15th Street, Augusta, Georgia 30912, USA |
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| Abstract: | Background DNA transposons have emerged as indispensible tools for manipulating vertebrate genomes with applications ranging from insertional mutagenesis and transgenesis to gene therapy. To fully explore the potential of two highly active DNA transposons, piggyBac and Tol2, as mammalian genetic tools, we have conducted a side-by-side comparison of the two transposon systems in the same setting to evaluate their advantages and disadvantages for use in gene therapy and gene discovery. |
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