Isolation of a chitin-binding lectin, with insecticidal activity in chemically-defined synthetic diets, from two wild brassica species with resistance to cabbage aphidBrevicoryne brassicae |
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Authors: | R A Cole |
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Institution: | (1) Horticulture Research International, Wellesbourne, CV359EF Warwick, UK |
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Abstract: | A lectin, with a chitin-binding domain and chitinase activity, is present in significant quantities in the wild brassica speciesB. fruticulosa andB. spinescens but at low levels in cultivated cabbage cv. Offenham Compacta. The lectin, purified >1000 fold after binding to chitin, migrated
on SDS-PAGE gels as a single band with a M
r
of 14.500. The amino acid composition of the lectin fromB. spinescens indicated high concentrations of asparagine/aspartic acid, glycine, leucine and serine in common with other chitin-binding
lectins with insecticidal and antifungal activities. Brassica lectin and the closely related agglutinin from wheatgerm and
nettle show significant insecticidal activity when presented toBrevicoryne brassicae in chemically-defined synthetic diets. |
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Keywords: | Brassica fruticulosa Brassica spinescens Brevicoryne brassicae lectin host-plant resistance chitin artificial diets chitinase |
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