一种新的用于提高lea3基因扩增特异性和产量的增强剂（英文）

在对目的DNA序列尤其是高GC含量的片段进行PCR扩增的时候,经常需要对一些试验条件进行优化。在一定条件下,二甲基亚砜、甲酰胺、甘油、NP-40和Tween20等可以在某种程度上提高PCR的特异性和效率。我们在对禾本科lea3基因进行分离克隆时发现了一种新的可以提高PCR产量和特异性的物质——极高热稳定单链结合蛋白（ETSSB）,研究发现在每50μlPCR反应体系中加入200ng的ETSSB,可以有效地抑制DNA片段的非特异性条带的产生,并可以提高目的片段的产量。

Effect of New Specificity and Yield Enhancer on Amplification of lea 3 Gene

Amplification of a target DNA by the polymerase chain reaction (PCR) often requires experimental optimization factors especially when the target sequence contains high content of GC. Dimethyl sulfoxide, formamide, glycerol, NP-40 and Tween20 are known to increase, the specificity and efficiency of PCR under certain conditions. In the amplification of lea3 gene in Gramineae, we found a new Extreme Thermostable Single-Stranded DNA Binding Protein (ET SSB) and improved yield of PCR. Addition of 200 ng of ET SSB per 50 μl reation decreased the formation of non-specific DNA fragments and increased the yield of specific PCR products.