A preliminary crystallographic study of isocitrate dehydrogenase from Azotobacter vinelandii |
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Authors: | E W Czerwinski P H Bethge F S Mathews A E Chung |
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Institution: | Department of Physiology and Biophysics Washington University School of Medicine 660 South Euclid Avenue St Louis, Mo. 63110, U.S.A.;Department of Life Sciences University of Pittsburgh Pittsburgh, Pa. 15261, U.S.A. |
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Abstract: | Large single crystals of isocitrate dehydrogenase from Azotobacter vinelandii have been grown by vapor diffusion from ammonium sulfate and phosphate solutions. The crystals are tetragonal, space group P42212 with cell dimensions , . There are two molecules of 80,000 molecular weight per asymmetric unit. Native data to 5.5 Å resolution have been collected on a diffractometer. A rotation function using data between 10 Å and 6 Å resolution indicates three possible orientations of the non-crystallographic 2-fold axis relating the two molecules. |
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