Cadmium-copper antagonism in the activation of periplasmic nitrous oxide reductase of copper-deficient cells fromPseudomonas stutzeri |
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Authors: | N Minagawa W G Zumft |
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Institution: | (1) Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Kaiserstraße 12, D-7500 Karlsruhe 1, Germany |
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Abstract: | Summary Copper-deficient cells ofPseudomonas stutzeri strain ZoBell synthesize catalytically inactive nitrous oxide (N2O) reductase which is activated by added Cu(II) in the absence of de novo protein synthesis. The apparentK
m for the activation process is 0.13 M. Activation is temperature-dependent and is inhibited by Cd(II)(K
i 1.27 M) and less strongly by Zn(II), Ni(II), and Co(II). The same metal ions at 20 M have little or no effect on N2O reduction of intact cells. Apo-N2O reductase of transposon Tn5-inducednos
– mutants with defective Cu-chromophore biosynthesis is not reactivated by Cu(II). N2O reductase of Cu-sufficient and Cu-deficient wild type, and ofnos
– mutants is localized in the periplasm, the latter providing the likely site of metal incorporation into the apoenzyme. |
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Keywords: | Nitrous oxide respiration Copper chromophore biosynthesis Cytochromeed
1 Denitrification Periplasmic enzyme Pseudomonas |
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