| 绿色木霉粗纤维素酶液水解壳聚糖的研究 |
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| 引用本文： | 常江波,余晓斌,谭秀花. 绿色木霉粗纤维素酶液水解壳聚糖的研究[J]. 工业微生物, 2011, 41(4): 26-30. DOI: 10.3969/j.issn.1001-6678.2011.04.006 |
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| 作者姓名： | 常江波 余晓斌 谭秀花 |
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| 作者单位： | 江南大学糖化学与生物技术教育部重点实验室,江苏,无锡,214122 |
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| 基金项目： | 高技术研究发展计划'863计划'，江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 利用自制绿色木霉粗纤维素酶液降解壳聚糖制备低聚壳聚糖.采用粘度法、乙酰丙酮法和还原糖浓度分析,研究了温度、pH值及反应时间等因素对壳聚糖水解程度和产物相对分子质量的影响,并采用质谱法对水解产物进行定性分析.结果表明,粗纤维素酶液水解壳聚糖作用的最适pH为5.0、最适反应温度为50 ℃、最适反应时间为12 h.粗纤维素酶...
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| 关 键 词： | 低聚壳聚糖 纤维素酶 质谱法 |
Studies on depolymerization of chitosan by cellulase |
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| CHANG Jing-bo,YU Xiao-bin,TAN Xiu-hua. Studies on depolymerization of chitosan by cellulase[J]. Industrial Microbiology, 2011, 41(4): 26-30. DOI: 10.3969/j.issn.1001-6678.2011.04.006 |
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| Authors: | CHANG Jing-bo YU Xiao-bin TAN Xiu-hua |
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| Affiliation: | (The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School Bioteehnology, Jiangnan University, Wuxl 214122, China) |
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| Abstract: | Chitosan oligosaccharide was obtained through the hydrolytic level of chitosan by ceUulase. The effects such as temperature, pH, and reaction time on depolymerization of chitosan were investigated using acetylaeetone method, viscosity method and reducing sugar concentration method, and the mass spectrometry was used to analyse hydrolysis product. The results showed that the optimal conditions for depolymerizing chitosan with cellulase were as follows： tem- perature 50 ℃, pH 5.0, reaction time 12 h. Chitosan could be hydrolyzed effectively by cellulase and the hydrolysis product had chitosan oligosaccharide with degree of polymerization of 1-7. |
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| Keywords: | chitosan oligosaccharide cellulase mass spectrometry |
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