The nucleotide sequences of some large ribonuclease T1 products from bacteriophage R17 ribonucleic acid |
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| Authors: | Peter G N Jeppesen |
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| Institution: | Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, U.K. |
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| Abstract: | A method of `fingerprinting' high-molecular-weight 32P-labelled RNA species, using a two-dimensional thin-layer-chromatographic separation of ribonuclease T1 digestion products, has been applied to RNA from the Escherichia coli bacteriophage R17. The `fingerprinting' technique, besides giving a unique pattern that can be used as a characterization of the RNA, has made it possible to isolate a number of the larger oligonucleotides and to determine their nucleotide sequences. |
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