Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin |
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Authors: | Wentworth M Ruban A V Horton P |
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Affiliation: | Robert Hill Institute for Photosynthesis Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, UK. |
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Abstract: | Non-photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xanthophyll cycle. A new in vitro model for this process has been developed. Purified light harvesting complexes above the detergent critical micelle concentration have a stable high fluorescence yield but a rapidly inducible fluorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was without effect, lutein and antheraxanthin induced a marginal response, whereas the violaxanthin analogue, auroxanthin, induced strong quenching. Quenching was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin-dependent alteration in the chlorophyll environment. |
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