Effect of lysine residues on the deamidation reaction of asparagine side chains |
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Authors: | Capasso S Balboni G Di Cerbo P |
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Institution: | Dipartimento di Scienze Ambientali, Seconda Università di Napoli, Caserta, Italy. capasso@chemna.dichi.unina.it |
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Abstract: | The effect of lysine residues on the deamidation reaction of the asparagine side chain has been studied on the peptide and on its lysine-acetylated derivative in a wide range of pH values. The amino acid sequence of these peptides is similar to the local sequence flanking the labile Asn-67 in RNAse A. The experimental data show that Lys influences both the deamidation rate and the relative yield of the two reaction products, i.e., the aspartic acid and beta-aspartic acid containing peptide. These effects are pH dependent and can be rationalized based on the mechanism previously proposed for the deamidation reaction via succinimide derivative. |
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