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Transformation of Brazilian eliteIndica-type rice (Oryza sativa L.) by electroporation of shoot apex explants |
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| Authors: | Vânia L Muniz de Péadua Rodrigo P Ferreira Larissa Meneses Nathaly Uchôa Méarcia Margis-Pinheiro Elisabeth Mansur |
| Institution: | 1. UFRJ, Instituto de Biologia, Dept. Genetica, Cidade Universitéaria, CP68011, CEP 21944-970, Rio de Janeiro, RJ, Brazil 3. UERJ, Instituto de Biologia, DBCG, Rua S?o Francisco Xavier 524 - PHLC/sl 505, Rio de Janeiro, RJ, Brazil |
| Abstract: | We have generated transgenic plants of a Brazilian elite Indica-type rice by electroporation of shoot apices. This approach avoids a callus phase and produces 0.4–13.8% resistant plants. Transgenic plantlets were transferred to soil a few weeks after explant electroporation. Root segments from plantlets obtained from transformation experiments with pAHC25 plasmid were GUS positive. Integration of the introduced gene into the genome was demonstrated by PPT and antibiotic screening as well as by PCR and Southern blot hybridization of genomic DNA isolated from R2 plantlets. |
| Keywords: | direct regeneration direct transformation HYG NPT PPT |
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