Detection of dilute antigen in large sample volumes using antibody-coated polyester cloth |
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| Authors: | Scott Boyd Hiroshi Yamazaki |
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| Institution: | (1) Department of Biology and Institute of Biochemistry, Carleton University, K1S 5B6 Ottawa, Ontario, Canada |
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| Abstract: | Summary A model antibody, goat anti-rabbit IgG antibody, was adsorbed onto a disk of polyester cloth and then fixed into a column apparatus. The macroporosity of the cloth allowed rapid immunoconcentration of a model antigen, rabbit IgG, by passing a large volume of the dilute antigen through the antibody-coated cloth. Such immunoconcentration permitted detection of the dilute antigen which otherwise would have gone undetected. |
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