| Abstract: | Cell cultures of Perilla frutescens, growing on Linsmaier and Skoog medium, released more anthocyanin with 40 to 50 g sucrose/l compared with the control of 30 g sucrose/l of medium. More proteins were also released into the medium with the higher sucrose concentration and the cell volume was smaller, suggesting that the higher osmotic pressure, caused by the high sucrose concentration, may cause these releases. The capacitance of the cultured cells indicated a difference in membrane structure between the cells cultivated with different sucrose concentrations, supporting the hypothesis that cell permeabilization is increased at higher sucrose concentrations.J.-J. Zhong is with the Research Institute of Biochemical Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China, G.-R. Xu is with the Department of Fermentation Technology, Wuxi Institue of Light Industry, Jiangsu 214036, China. T. Yoshida is with the International Center of Cooperative Research in Biotechnology, Faculty of Engineering. Osaka University, Osaka 565, Japan. |
