Changes in levels of amino acid acceptors in tRNA from Escherichia coli grown under various conditions |
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Authors: | R Chase G M Tener I C Gillam |
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Institution: | 1. Departments of Pharmacology and Medicine, Biochemistry, University of Washington School of Medicine Seattle, Washington 98195, USA;2. Sloan-Kettering Institute, New York, New York 10023 USA |
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Abstract: | Active enzyme sedimentation of five asparaginase and glutaminase-asparaginase enzymes with antitumor activity was studied. The catalytically active species of each enzyme appeared to have a molecular weight greater than 100,000 g/mole. Gel filtration and disc gel electrophoresis confirmed the absence of catalytically active smaller species. |
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