Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome |
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| Authors: | A. Bihlmater,U. Rö mling,T. F. Meyer,B. Tü mmler,C. P. Gibbs |
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| Affiliation: | Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Spemannstraße 34, D-7400 Tübingen, Germany.;Medizinische Hochschute Hannover, Zentrum Biochemie, Institut für Biophysikatische Chemie, D-3000 Hannover 61, Germany. |
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| Abstract: | A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one-and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33Mb±35kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization. |
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