Effect of hydroxyurea on subcellular activities of thymidine kinase in developing and aging rat brain |
| |
Authors: | V. Prabhakar Kalluri Subba Rao |
| |
Affiliation: | (1) Neurochemistry Laboratory, School of Life Sciences, University of Hyderabad, 500 134 Hyderabad, India |
| |
Abstract: | Hydroxyurea, when injected intraperitoneally at a dose of 1 mg/g body weight, inhibited thymidine kinase activity in developing rat cerebrum (16-day-embryonic) and cerebellum (7-day-postnatal) within a few hours of administration. The inhibition was time-dependent and both cytosolic and mitochondrial thymidine kinases were affected. Under the same conditions, the activities of certain other enzymes concerned with DNA metabolism,viz., DNA polymerase, and acid and alkaline DNases were not inhibited. Further, the addition of hydroxyureain vitro had no effect on the activity of any of the enzymes studied. However, similar treatment given to 2-year-old rat failed to exert any inhibition on either the mitochondrial or soluble thymidine kinase activities in grey and white matter regions of cerebrum and cerebellum. It is inferred that hydroxyurea, apart from its already known effect on ribonucleotide reductase of replicating cells, also affects thymidine kinase. |
| |
Keywords: | Hydroxyurea mitochondrial and cytosolic thymidine kinases DNA polymerase acid and alkaline DNases |
本文献已被 SpringerLink 等数据库收录! |
|