<Emphasis Type="Italic">In vitro</Emphasis> direct organogenesis and regeneration of <Emphasis Type="Italic">Medicago sativa</Emphasis> |
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Authors: | J J Li YM Wu T Wang J X Liu |
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Institution: | (1) College of Animal Sciences, Zhejiang University, Hangzhou, 310029, P.R. China |
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Abstract: | A rapid and efficient plant regeneration protocol for a wide range of alfalfa genotypes was developed via direct organogenesis. Through a successive excision of the newly developed apical and axillary shoots, a lot of adventitious
buds were directly induced from the cotyledonary nodes when hypocotyl of explants were vertically inserted into modified Murashige
and Skoog (MS) medium supplemented with 0.025 mg dm−3 thidiazuron (TDZ) and 3 mg dm−3 AgNO3. When the lower part of shoots excised from explants were immersed into the liquid medium with 1.0 mg dm−3 α-naphthaleneacetic acid (NAA) for 2 min, and then transferred to hormone free half-strength MS medium, over 83.3 % of the
shoots developed roots, and all plantlets could acclimatize and establish in soil. The protocol has been successfully applied
to eight genotypes, with regeneration frequencies ranging from 63.8 to 82.5 %. |
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Keywords: | Additional" target="_blank">Additional alfalfa cotyledonary node mature embryo silver nitrate thidiazuron vitrification |
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