A preliminary study of chromium distribution in chromium-rich brewer's yeast cell by NAA |
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| Authors: | Ding W J Qian Q F Hou X L Feng W Y Chen C Y Chai Z F Zhang B R Wang K |
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| Institution: | (1) Institute of High Energy Physics and Laboratory of Nuclear Analysis Techniques, Chinese Academy of Sciences, P.O. Box 918, 100039 Beijing, People’s Republic of China;(2) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, People’s Republic of China;(3) Chinese Institute of Atomic Energy, 102413 Beijing, People’s Republic of China |
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| Abstract: | The purpose of this study was to assess the chromium (Cr) distribution in chromium-rich brewer’s yeast cell. The chromium
concentrations in the cell wall and protoplast fractions of the chromium-rich yeast were determined by neutron activation
analysis (NAA). Moreover, the combined state of chromium and amino acid content in the Cr-rich brewer’s yeasts was analyzed
and measured. The experimental results indicate that the introduction of water-soluble chromium (III) salt as a component
of the culture medium for yeasts results in a substantial amount of chromium absorbed through the cell wall by the yeast,
among which 80.9% are accumulated in the protoplast. It implies that, under optimal conditions, yeasts are capable of accumulating
large amounts of chromium and incorporating chromium into organic compounds. |
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| Keywords: | Brewer’ s yeast chromium enrichment cell wall protoplast neutron activation analysis |
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