Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3 |
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| Authors: | M Kriechbaum H J Heilmann F J Wientjes M Hahn K D Jany H G Gassen F Sharif G Alaeddinoglu |
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| Institution: | Institut für Biochemie, Technische Hochschule Darmstadt, FRG. |
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| Abstract: | A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing. |
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