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Genetic analysis of the structure and function of RNase P fromE. coli |
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| Authors: | Hideaki Shiraishi Yoshiro Shimura |
| Affiliation: | (1) Department of Chemistry, Faculty of Science, Kyoto University, 606 Kyoto, Japan;(2) Department of Biophysics, Faculty of Science, Kyoto University, 606 Kyoto, Japan;(3) Present address: Biomolecular Engineering Research Institute, 6-2-3 Furuedai, 565 Suita, Osaka, Japan |
| Abstract: | A brief review of the genetic studies on ribonuclease P (RNase P) fromEscherichia coli is presented. Temperature-sensitive mutants ofE. coli defective in tRNA processing were isolated by screening cells which were unable to synthesize a suppressor tRNA at restrictive temperature. Structural analysis of accumulated tRNA precursors showed that the isolated mutants were defective in RNase P activity. Analyses of the mutants revealed that the enzyme is essential for the synthesis of all tRNA molecules in cells and that the enzymes consists of two subunits. Analyses of the isolated mutants revealed a possible domain structure of the RNA subunit of the enzyme.Abbreviations E. coli Escherichia coli - RNase P ribonuclease P |
| Keywords: | Escherichia coli M1 RNA Ribonuclease P Ribozyme RNA processing tRNA |
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