One-year follow-up of anti-Leishmania antibody concentrations in serum and saliva from experimentally infected dogs |
| |
| Affiliation: | 1. Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Espinardo, Murcia, Spain;2. Molecular Biology Department, Instituto de Parasitología y Biomedicina “López Neyra”, Consejo Superior de Investigaciones Científicas, Granada, Spain;1. Instituto de Ciencia de Materiales de Sevilla (CSIC-Univ. Sevilla), Avda. Américo Vespucio 49, 41092 Sevilla, Spain;2. Departamento de Física de la Materia Condensada, Universidad de Sevilla, Apdo. 1065, 41080 Sevilla, Spain;1. Unidad Regional de Medicina Tropical, Servicio de Microbiología, Hospital Universitario Virgen de la Arrixaca, Murcia, Spain;2. Departamento de Genética y Microbiología, Universidad de Murcia, Spain;3. Instituto de Parasitología y Biomedicina López-Neyra, Consejo Superior de Investigaciones Científicas (IPBLN-CSIC), Granada, Spain;4. Centro Nacional de Medicina Tropical, Instituto de Salud Carlos III, Madrid, Spain;1. Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea;2. Department of Thoracic and Cardiovascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea;3. Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea;4. Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea;1. Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Alberta, Canada;2. Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Scotland, United Kingdom;3. Wellcome Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, United Kingdom;4. Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, United Kingdom;5. University of Edinburgh, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, United Kingdom;1. School of Life Sciences, University of KwaZulu-Natal, Durban, Westville, South Africa;2. Agricultural Research Council-Onderstepoort Veterinary Research, Onderstepoort 0110, South Africa;3. The Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa;4. Department of Agriculture and Animal Health, College of Agriculture & Environmental Sciences, University of South Africa, South Africa;5. Department of Life and Consumer Sciences, College of Agriculture & Environmental Sciences, University of South Africa, South Africa;1. School of Biological Sciences, Queen’s University Belfast, Northern Ireland, United Kingdom;2. International Institute of Tropical Agriculture, Kenya |
| |
| Abstract: | The quantification of anti-Leishmania antibodies in serum and saliva by a time-resolved immunofluorometric assay is useful for the diagnosis and treatment monitoring of dogs with clinical leishmaniasis. We compared the kinetics of anti-Leishmania IgG2 and IgA antibodies in serum and saliva from 11 Beagle dogs experimentally infected with Leishmania infantum. Most dogs showed detectable concentrations of anti-Leishmania IgG2 earlier in serum (between 3 and 4 months p.i.) than in saliva (between 4 and 6 months p.i.). Overall, a high correlation between concentrations of anti-Leishmania IgG2 in serum and saliva (r = 0.853; P < 0.0001) was observed. The quantification of anti-Leishmania IgA showed less diagnostic value than IgG2, since detectable amounts of IgA were not observed in the saliva of four dogs and in the serum of one dog. In addition, a very low correlation between anti-Leishmania IgA in serum and saliva (r = 0.289; P < 0.001) was observed. Our results indicate that the antibodies against L. infantum in saliva appear approximately 1 month later than in serum, and suggest that there is a threshold for the passing of immunoglobulins from serum to saliva in dogs. These facts should be taken into consideration for a proper interpretation of saliva assays for quantification of antibodies. |
| |
| Keywords: | Dog Experimental infection Saliva Serum |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
