PEGylated click polypeptides synthesized by copper-free microwave-assisted thermal click polymerization for selective endotoxin removal from protein solutions |
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| Authors: | Guo Jinshan Meng Fanbo Li Xiaoyuan Wang Mingzhe Wu Yanjuan Jing Xiabin Huang Yubin |
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| Institution: | State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China. |
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| Abstract: | PEGylated click polypeptides (PEG-CPs) containing α-amino side groups as well as PEG segments are designed for selective endotoxin removal from protein solutions. The PEG-CPs are synthesized via copper-free thermal click copolymerization from aspartic (or glutamic) acid-based dialkyne and diazide monomers (containing free amino side groups) and alkyne-terminated mPEGs or dialkyne-terminated PEGs. Microwave-assisting technology is introduced into thermal click chemistry to improve the reaction efficiency. The monomers and polymers are fully characterized using NMR, XPS, and MALDI-TOF MS. After immobilizing the PEGylated click polypeptides onto polystyrene microspheres, the adsorbents exhibit good endotoxin removal selectivity from BSA solutions. |
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