Differential effect of pH on the density and volume of rat reticulocytes and erythrocytes |
| |
Authors: | Harry Walter Eugene J Krob Rosalinda B Wenby Herbert J Meiselman |
| |
Institution: | 1. Laboratory of Chemical Biology, Veterans Administration Medical Center, 90822, Long Beach, CA 2. Department of Physiology, University of California, 92717, Irvine, CA 3. Department of Biophysics, University of Southern California School of Medicine, 90033, Los Angeles, CA
|
| |
Abstract: | Rats were injected with59Fe-ferrous citrate and bled thereafter at different times (16 h to 49 d). This gave rise to red cell populations in which
cells corresponding in age to the time elapsed between injection and bleeding were labeled. The anticoagulant used was either
acid-citrate-dextrose (ACD) with a pH adjusted to 7.3 or ACD (pH 5.1). Final pH of the collected blood was about 7.2–7.4 in
the former case and 6.4–6.7 in the latter. Red cells were then centrifuged (5) and approximately 7–10% of the packed cells
from the top and 7–10% from the bottom of the cell column collected. When reticulocytes are the predominant labeled red cell
population, as in blood obtained for about 24 h after isotope injection, a fractionation of these cells and mature erythrocytes
is in evidence only when blood is collected at the higher pH. Thus, at pH 7.2–7.4 ratios of specific radioactivities of cells
in top fraction/cells in an unfractionated sample are about 3, whereas at pH 6.4–6.7, the analogous ratios are 1 or less.
These differences in specific activity ratios, as a function of pH at collection, virtually disappear after about 4 d following
isotope injection.
The lower pH is known to increase the volume and decrease the density of mature red blood cells. The marked effect of pH on
cellular fractionation could be correlated with the smaller change in rat reticulocyte density and volume in acid medium.
At pH 6.4–6.7, the densities of mature erythrocytes and reticulocytes are so close that their physical separation by centrifugation
is not feasible. |
| |
Keywords: | Index Entries" target="_blank">Index Entries Reticulocytes erythrocytes red blood cells rats fractionation centrifugation 59-Fe-ferrous citrate |
本文献已被 SpringerLink 等数据库收录! |
|