Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.) |
| |
| Authors: | Y. Mano B. E. Sayed-Tabatabaei A. Graner T. Blake F. Takaiwa S. Oka T. Komatsuda |
| |
| Affiliation: | (1) Department of Biotechnology, National Institute of Agrobiological Resources, Kannondai, Tsukuba 305-8602, Japan Fax: +81 298 38 8397, E-mail: mano@abr.affrc.go.jp, JP;(2) Institute for Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466 Gatersleben, Germany, DE;(3) Department of Plant, Soil and Environmental Sciences, Montana State University, Bozeman, MT 59717, USA, US |
| |
| Abstract: | In order to identify sequence-tagged sites (STSs) appropriate for recombinant inbred lines (RILs) of barley cultivars ‘Azumamugi’ × ‘Kanto Nakate Gold’, a total of 43 STS primer pairs were generated on the basis of the terminal sequences of barley restriction fragment length polymorphism (RFLP) clones. Forty one of the 43 primer pairs amplified PCR products in Azumamugi, Kanto Nakate Gold, or both. Of these, two showed a length polymorphism and two showed the presence or absence of polymorphism between the parents. PCR products of the remaining 37 primers were digested with 46 restriction endonucleases, and polymorphisms were detected for 15 primers. A 383.6-cM linkage map of RILs of Azumamugi×Kanto Nakate Gold was constructed from the 19 polymorphic STS primer pairs (20 loci) developed in this study, 45 previously developed STS primer pairs (47 loci), and two morphological loci. Linkage analysis and analysis of wheat-barley chromosome addition lines showed that with three exceptions, the chromosome locations of the STS markers were identical with those of the RFLP markers. Received: 4 August 1998 / Accepted: 8 October 1998 |
| |
| Keywords: | Hordeum vulgare Linkage map Recombinant inbred Sequence-tagged site (STS) |
| 本文献已被 SpringerLink 等数据库收录！ |
|
