ON ARABINOSE AS A CONSTITUENT OF HYALURONIC ACID FROM BOVINE BRAIN |
| |
| Authors: | R. L. Katzman |
| |
| Affiliation: | Laboratory for Carbohydrate Research, Departments of Biological Chemistry and Medicine, Harvard Medical School at the Massachusetts General Hospital, Boston, Massachusetts 02114 |
| |
| Abstract: | (1) Wardi , Allen , Turner and Stary (1966) and Margolis (1967) have reported that arabinose is a component of hyaluronic acid from mammalian brain. (2) In the present study, total acidic polysaccharide and hyaluronic acid fractions were isolated from lipid-extracted and proteolysed bovine brain by precipitation with cetyltri-methylammonium bromide. These fractions were analysed for arabinose by paper chromatography of deionized hydrolysates and by gas-liquid chromatography of per(trimethylsilyl)ated methanolysates. (3) Two pentoses, xylose and ribose, were detected. Arabinose was analytically undetectable in both polysaccharide fractions, but was easily detected in a control polysaccharide containing 0-1% (w/w) arabinose. Arabinose, if present in hyaluronic acid from bovine brain, constitutes less than 0.1 mol per mol of hyaluronic acid (molecular weight 1.5 x 106 daltons). |
| |
| Keywords: | |
|
|
