H-2-linked genes determine the level of the primary in vitro anti-Mls response |
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Authors: | Stuart Macphail Osias Stutman |
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Institution: | (1) Immunology Program, Memorial Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, 10021 New York, New York |
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Abstract: | The level of cell proliferation and interleukin-2 (IL-2) production observed in an anti-Mls mixed lymphocyte reaction between spleen cells from H-2 compatible, Mls incompatible mouse strains is determined by the H-2 haplotype of the mouse combination. Thus, while AKR (H-2
k) spleen cells stimulated strong M1sa responses in H-2k responder cells, AKR H-2b spleen cells stimulated no or negligible M1sa responses in responder cells from H-2
bmouse strains. This effect was observed at the levels of IL-2 production and cell proliferation. The magnitude of the response observed using F1 (H-2
k/H-2
b) responder cells was found to be a function of stimulator rather than responder cells. The poor stimulatory capacity of AKRH-2
bspleen cells was also shown not to be due to the loss of the stimulatory Mls
aallele during the construction of the congenic strain from AKR and C57BL/6 parental strains. Using stimulator cells from a second series of congenic mice, we found H-2
b(strain DLLP) again to represent a poorly Mlsa stimulatory H-2 haplotype. In addition, H-2q (DBA/1) cells displayed very poor Mlsa stimulatory potential while H-2d (D1.C) cells were efficient Mlsa stimulators. Again the effect was shown to be at the level of the stimulator cells. In toto, our findings indicate that the H-2
kand H-2
dhaplotypes encode strong Mlsa stimulatory potential while the H-2
band H-2
qhaplotypes determine poor Mlsa stimulatory potential in primary in vitro responses, measured as cell proliferation and IL-2 production.Abbreviations used in this paper: CTL
cytotoxic T lymphocyte
- IL-1
interleukin-1
- IL-2
interleukin-2
- MLR
mixed lymphocyte reaction
- NMS
normal mouse serum |
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