Cytosolic phospholipase A2-driven PGE2 synthesis within unsaturated fatty acids-induced lipid bodies of epithelial cells |
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Authors: | Luciana S Moreira Bruno Piva Luciana B Gentile Fabio P Mesquita-Santos Heloisa D'Avila Clarissa M Maya-Monteiro Patrícia T Bozza Christianne Bandeira-Melo Bruno L Diaz |
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Institution: | 1. Laboratório de Inflamação, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Bloco C, Room C1-024, Av. Carlos Chagas Filho 373, Rio de Janeiro, RJ 21941-902, Brazil;2. Laboratório de Imunofarmacologia, Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil |
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Abstract: | Cytoplasmic lipid bodies (also known as lipid droplets) are intracellular deposits of arachidonic acid (AA), which can be metabolized for eicosanoid generation. PGE2 is a major AA metabolite produced by epithelial cells and can modulate restoration of epithelium homeostasis after injury. We studied lipid body biogenesis and their role in AA metabolic pathway in an epithelial cell line derived from normal rat intestinal epithelium, IEC-6 cells. Lipid bodies were virtually absent in confluent IEC-6 cells. Stimulation of confluent IEC-6 cells with unsaturated fatty acids, including AA or oleic acid (OA), induced rapid lipid body assembly that was independent on its metabolism to PGE2, but dependent on G-coupled receptor-driven signaling through p38, PKC, and PI3K. Newly formed lipid bodies compartmentalized cytosolic phospholipase (cPL)A2-α, while facilitated AA mobilization and synthesis of PGE2 within epithelial cells. Thus, both lipid body-related events, including highly regulated biogenesis and functional assembly of cPLA2-α-driven enhanced AA mobilization and PGE2 production, may have key roles in epithelial cell-driven inflammatory functions, and may represent relevant therapeutic targets of epithelial pathologies. |
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Keywords: | Lipid droplet Epithelial cell cPLA2-α PGE2 Arachidonic acid Oleic acid p38 MAP kinase |
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