Incorporation of exogenous glycosphingolipids in plasma membranes of cultured hamster cells and concurrent change of growth behavior |
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| Authors: | R A Laine S Hakomori |
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| Institution: | Departments of Pathobiology and Microbiology School of Public Health and School of Medicine University of Washington, Seattle, Washington 98195 USA |
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| Abstract: | Globoside added to culture medium was taken up by NIL cells and accumulated as a component of plasma membrane. This was evidenced by the recovery of 3H-labelled globoside from plasma membrane fractions and by the higher chemical quantity of globoside found in NIL cells cultured in medium containing globoside. Concomitantly the following changes in growth behavior were manifested: a reduction in growth rate due to an extended prereplicative phase and a reduced saturation density which may result from changed adhesive properties of cells. |
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| Keywords: | globoside a trivial name for a ceramide tetrasaccharide whose structure was established as GalNAcβ1→3Galα1→4Galβ1→4Glc→Ceramide (1 2) TLC thin-layer chromatography |
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