N-carboxyacyl-N-acylchitosan gels as novel media for gel chromatography

Three partially substituted N-carboxyacyl and six N-carboxyacyl-N-acyl derivatives of chitosan were prepared and their practical use as media for gel chromatography was examined. N-(3′-Carboxy-2′-propenoyl)-N-stearoyl-chitosan gel was a relatively good medium for gel chromatography (solvent, water), and had a wide fractionation range (MW = 2 × 10⁴?6 × 10⁵). Its chromatographic properties were compared with those of N-methylene-chitosan gel (solvent, 0·5 m NaCl).