Extent of β-glucan chain elongation by ryegrass (Lolium multiflorum) enzymes |
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Authors: | Robert J. Henry Bruce A. Stone |
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Affiliation: | Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia |
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Abstract: | The chain length and linkage composition of water-soluble β-glucans produced in vitro from UDP-[14C] glucose by ryegrass membranes was determined by methylation analysis. The methylated β-glucans were acid-hydrolysed and the partially methylated sugar products reduced to the corresponding methylated alditols. Mass spectra were recorded for the permethylated alditols following their separation by reversed-phase h.p.l.c. 64% of the radiolabelled β-glucosyl residues were 3-substituted, 33% were 4-substituted and 3% were non-reducing terminal residues indicating that the average degree of polymerization of the radiolabelled sequences was 33. This result demonstrates that substantial elongation of β-glucan chains was occurring in vitro and that chain elongation was from the non-reducing end. |
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