Factors influencing renal cryopreservation. II. Toxic effects of three cryoprotectants in combination with three vehicle solutions in nonfrozen rabbit cortical slices |
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| Authors: | P Clark G M Fahy A M Karow |
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| Institution: | 1. Department of Pharmacology, Medical College of Georgia, Augusta, Georgia 30912, U.S.A.;1. Blood Services Laboratories, Cryobiology Laboratories, American National Red Cross, 9312 Old Georgetown Road, Bethesda, Maryland 20014 U.S.A. |
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| Abstract: | The K+]/Na+] ratio of rabbit renal cortical slices was used to examine, at 25 degrees C, the effects on viability of three cryoprotectant agents (CPA) (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) in combination with three vehicle solutions (Krebs-Henseleit (K-H), solution A, and RPS-2). Viability assessment by K+]/Na+] for all test solutions was made after incubating the slices in modified Cross-Taggart solution (C-T). With K-H and solution A, all concentrations of ethylene glycol and glycerol resulted in lowered ratios, whereas with Me2SO, concentrations greater than 1.4 M are required to reduce K+]/Na+]. With RPS-2 no decrease in the ratios was found until concentrations greater than 2.8 M were reached for all three CPAs. Binding of Me2SO to albumin, studied using 14C]Me2SO, was inhibited by RPS-2 when compared to K-H. Introduction and removal of Me2SO at 10 degrees C allowed an improvement in viability, at higher Me2SO concentrations, as compared to 25 degrees C. |
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| Keywords: | To whom reprint requests should be addressed |
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