Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160 |
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Authors: | Geddes C C Mullinnix M T Nieves I U Peterson J J Hoffman R W York S W Yomano L P Miller E N Shanmugam K T Ingram L O |
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Affiliation: | a Department of Microbiology and Cell Science, University of Florida, Box 110700, Gainesville, FL 32611, United States b Department of Agriculture and Biological Engineering, University of Florida, Box 110570, Gainesville, FL 32611, United States |
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Abstract: | Hexose and pentose sugars from phosphoric acid pretreated sugarcane bagasse were co-fermented to ethanol in a single vessel (SScF), eliminating process steps for solid-liquid separation and sugar cleanup. An initial liquefaction step (L) with cellulase was included to improve mixing and saccharification (L + SScF), analogous to a corn ethanol process. Fermentation was enabled by the development of a hydrolysate-resistant mutant of Escherichia coli LY180, designated MM160. Strain MM160 was more resistant than the parent to inhibitors (furfural, 5-hydroxymethylfurfural, and acetate) formed during pretreatment. Bagasse slurries containing 10% and 14% dry weight (fiber plus solubles) were tested using pretreatment temperatures of 160-190 °C (1% phosphoric acid, 10 min). Enzymatic saccharification and inhibitor production both increased with pretreatment temperature. The highest titer (30 g/L ethanol) and yield (0.21 g ethanol/g bagasse dry weight) were obtained after incubation for 122 h using 14% dry weight slurries of pretreated bagasse (180 °C). |
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Keywords: | Cellulase Lignocellulose SSF L + SScF Ethanol |
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