Simultaneous detection of mRNA and protein stem cell markers in live cells |
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Authors: | Won Jong Rhee Gang Bao |
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Institution: | (1) Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA |
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Abstract: | Background Biological studies and medical application of stem cells often require the isolation of stem cells from a mixed cell population,
including the detection of cancer stem cells in tumor tissue, and isolation of induced pluripotent stem cells after eliciting
the expression of specific genes in adult cells. Here we report the detection of Oct-4 mRNA and SSEA-1 protein in live carcinoma
stem cells using respectively molecular beacon and dye-labeled antibody, aiming to establish a new method for stem cells detection
and isolation. |
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Keywords: | |
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