| 猪脑突触质膜糖皮质激素膜结合位点的测定 |
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| 引用本文： | 傅红,郭佐,陈宜张. 猪脑突触质膜糖皮质激素膜结合位点的测定[J]. 生理学报, 1992, 44(2): 170-174 |
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| 作者姓名： | 傅红 郭佐 陈宜张 |
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| 作者单位： | 第二军医大学生理学教研室，第二军医大学神经科学实验室 上海 200433，上海 200433 |
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| 摘 要： | 利用放射配体结合法,测定了猪脑六个不同脑区突触质膜糖皮质激素膜结合位点(GCM-BS)的最大结合量(Bmax)和平衡解离常数(Kd)。结果表明:猪的大脑、小脑、中脑、丘脑、下丘脑和尾状核的突触质膜GCMBS的Kd都在200nmol/L左右,提示它们对[~3H]皮质酮的亲和力基本相同;而各脑区内GCMBS的Bmax相差较大,从218.98fmol/mg蛋白至486.95fmol/mg蛋白不等,说明不同脑区内所含有的GCMBS的数量不同。
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| 关 键 词： | 突触质膜 皮质酮 糖皮质激素膜结合位点 |
ESTIMATION OF GLUCOCORTICOID MEMBRANE BINDING SITES IN SYNAPTIC PLASMA MEMBRANEISOLATED FROM PORCINE BRAIN |
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| FU HONG,GUO ZUO,CHEN YI-ZHANG Laboratory of Neuroscience. ESTIMATION OF GLUCOCORTICOID MEMBRANE BINDING SITES IN SYNAPTIC PLASMA MEMBRANEISOLATED FROM PORCINE BRAIN[J]. Acta Physiologica Sinica, 1992, 44(2): 170-174 |
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| Authors: | FU HONG GUO ZUO CHEN YI-ZHANG Laboratory of Neuroscience |
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| Affiliation: | Department of Physiology, Second Military Medical University, Shanghai. |
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| Abstract: | Using radioligand binding assay, we measured the B_(max) and Kd of glucocor-ticoid membrane binding sites (GCMBS) in the synaptic plasma membranes isolat-ed from different regions of porcine brain. The Kds of GCMBS in cerebrum,cerebellum, midbrain, thalamus, nuclei caudatus and hypothalamus are all around200 nmol/L. The B_(max) of GCMBS in the mentioned brain regions ranges from218.98 fmol/mg to 486.95 fmol/mg protein. |
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| Keywords: | synaptic plasma membrane corticosterone glucocorticoid membrane blading sites |
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