Comparison between levels of trace elements in normal and cancer inoculated mice by XRF and PIXE |
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Authors: | H Feldstein Y Cohen C Shenberg A Klein M Kojller W Maenhaut J Cafmeyer R Cornelis |
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Institution: | (1) SOREQ NRC, 81800 Yaune, Israel;(2) Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel;(3) Institute for Nuclear Sciences, RUG, Proeftuinstraat 86, B 9000 Gent, Belgium |
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Abstract: | Determination of Rb, Br, Se, Zn, Cu, Fe, and Br/Rb ratio in tissues of mice inoculated with colon and melanoma cancer cells
is described. A group of 19 Balb/c mice inoculated with C26 colon carcinoma, 4 C57B1/6 mice inoculated with B16 melanoma,
and 13 control mice of both kinds were under investigation. The study was conducted on samples of blood, liver, kidneys, colon,
and skin, and the trace element levels in normal and inoculated mice were compared. The inoculation was by subcutaneous injection
either at the back or intrafootpad. The blood samples were taken 1, 2, and 3 wk after inoculation, and after 4 wk all the
animals were sacrificed. Two nondestructive, complementary analytical methods were used: a modified X-ray fluorescence (XRF)
for solid tissue and particleinduced X-ray emission (PIXE) for blood samples. The detection limit (DL) in the PIXE method
was 0.35 (μg/g dry wt in 600 s counting time and in XRF, 1 μg/g dry sample for Rb, Br, Se and Zn and 2 μg/g for Cu and Fe
in 200 s counting time. In all the cases studied, cancerous tissue developed at the site of the injection, and a significant
difference in the trace element levels was observed between tissue samples obtained from normal and inoculated mice. The most
pronounced effect was an increase in Rb level in the tumor by a factor ranging between 4 and 10 relative to normal tissue,
with a corresponding decrease in the Br/Rb ratio (p < 0.05). Smaller changes were found in the Br, Se, Zn, and K levels. The changes in trace element levels in the inner organs
were much smaller and seem to be influenced by the site of injection. |
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Keywords: | Trace elements mice cancer XRF PIXE |
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