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Construction and characterisation of a BAC library from Arabidopsis halleri: Evaluation of physical mapping based on conserved synteny with Arabidopsis thaliana
Authors:Eric Lacombe  Magalie Cossegal  Marie Mirouze  Thibaud Adam  Fabrice Varoquaux  Stphanie Loubet  Pietro Piffanelli  Michel Lebrun  Pierre Berthomieu
Institution:

aLaboratoire de Biochimie & Physiologie Moléculaire des Plantes, UMR Université Montpellier 2-CNRS-INRA-Montpellier SupAgro, 2 Place Viala, 34060 Montpellier Cedex 01, France

bCIRAD, UMR 1096, Avenue Agropolis, TA40/03, 34398 Montpellier Cedex 5, France

Abstract:We constructed and characterized the first large-insert DNA BAC library for Arabidopsis halleri, a close relative of Arabidopsis thaliana. Double size selection of high molecular weight DNA was performed to increase the average insert size. The BAC library consists of 6128 clones of which 87% have an insert size above 125 kb. Organellar DNA contamination is estimated to 1.4%. The coverage of the library is equivalent to 4.5 times the haploid genome (250 Mb), indicating the library is suitable for almost any application. We explored the possibility of generating a physical map of A. halleri using the high conserved synteny existing between this species and A. thaliana. A set of unigenes separated by 50 kb in a 850 kb region of A. thaliana chromosome II was used to probe the library. The A. halleri BAC clones isolated with these probes were grouped into two contigs. Analysis of BAC-end sequences revealed that the two A. halleri genomic contigs were highly colinear with the A. thaliana genome. Therefore, the exploitation of the conserved synteny existing between the two species will greatly facilitate the construction of a raw full physical map of A. halleri.
Keywords:Arabidopsis halleri  BAC library  Physical mapping  Conserved synteny
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