Re-engineering Escherichia coli for ethanol production |
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Authors: | L P Yomano S W York S Zhou K T Shanmugam L O Ingram |
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Institution: | (1) Department Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA |
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Abstract: | A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH
and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and adhB) into the chromosome. By selecting for fermentative growth in mineral salts medium containing xylose, a highly productive
strain was isolated in which the ethanol cassette had been integrated behind the rrlE promoter, designated strain LY160 (KO11, Δfrd::celY
Ec
ΔadhE ΔldhA, ΔackA
lacA::casAB
Ko
rrlE::(pdc
Zm
-adhA
Zm
-adhB
Zm
-FRT-rrlE) pflB
+
). This strain fermented 9% (w/v) xylose to 4% (w/v) ethanol in 48 h in mineral salts medium, nearly equal to the performance
of KO11 with Luria broth. |
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Keywords: | Betaine E coli Ethanol Lactate Osmotic stress |
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