Induction of shape abnormality and unscheduled DNA synthesis by arecoline in the germ cells of mice

The ability of arecoline, an alkaloid of betel nut, to induce abnormality in the shape of sperm heads and unscheduled DNA synthesis (UDS) in the early spermatid stages of Swiss albino mice was studied. Treatment of mice with arecoline at the dose levels of 20, 40 and 80 mg/kg elicited dose-related increase in the number of abnormal sperm heads, as well as the unscheduled incorporation of 3H]thymidine into the DNA of early spermatids. Such increase in the production of abnormally shaped sperms and UDS response of the early spermatids following arecoline treatment expressed its genotoxic potential in the mouse germ cells.