Assembly and structure of neurofilaments isolated from bovine spinal cord |
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Authors: | Xiangjun Tong Jianguo Chen Jie Liu Shijin Pang Zhonghe Zhai |
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Institution: | 1. College of Life Sciences, Peking University, 100871, Beijing, China 2. Beijing Laboratory of Vacuum Physics, Institute of Physics, Chinese Academy of Sciences, 100080, Beijing, China
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Abstract: | Neurofilaments (NFs) are neuron-specific intermediate filaments. The NFs were isolated from bovine spinal cord by differential
centrifugation. The NFs were detected with electron microscopy and scanning tunneling microscopy (STM). Under STM, two kinds
of sidearm of NFs were revealed: one was short, the other was long. They were arrayed along the 10-nm width core filaments
one by one. The intervals between two adjacent long sidearms or two short sidearms were 20–22 nm, while those between two
adjacent long and short sidearms were 10–11 nm. It was proposed that the rod domain of NF triplet proteins was 3/4-staggered.
The assembly properties of NF triplet proteins were also studied. Immuno-colloidal-gold labeling assay showed that NF-M and
NF-H are able to co-assemble into long filaments with NF-L. NF-M and NF-H can also co-constitute into winding filaments. |
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