Enzymatic determination of serum-free fatty acids: a colorimetric method |
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Authors: | S Shimizu Y Tani H Yamada M Tabata T Murachi |
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Affiliation: | 1. Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan;2. Department of Clinical Science, Kyoto University Hospital, Kyoto 606, Japan |
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Abstract: | A simple and sensitive enzymatic method is described for the determination of serumfree fatty acids. The method is based on the activation of free fatty acids by acyl-CoA synthetase (EC 6.2.1.3). The reaction is followed as the production of hydrogen peroxide by using acyl-CoA oxidase/peroxidase/4-aminoantipyrine/phenol system. Results on human sera correlate well with those obtained by our previously described enzymatic method (Shimizu et al. (1979) Anal. Biochem.98, 341–345) and the chemical colorimetric method. |
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