Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene. |
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Authors: | S D Lupton L L Brunton V A Kalberg R W Overell |
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Affiliation: | Department of Gene Therapy, Immunex Corporation, Seattle, Washington 98101-2977. |
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Abstract: | The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity. |
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